Fluorescent Organic Nanoparticles Constructed by a Facile “Self-Isolation Enhanced Emission” Strategy for Cell Imaging

To achieve the highly emissive features and overcome the troublesome photobleaching for fluorescent organic molecules, a facile and versatile strategy named “self-isolation enhanced emission (SIEE)” was developed to prevent the π–π stacking of organic fluorophores by linking alkyl chains on their conjugated backbones. As a proof-of-concept, one or two octyl groups were grafted onto the backbone of 4,7-di(thiophen-2-yl)benzo[c][1,2,5]thiadiazole (termed as DTBT-0), resulting in two different molecules, termed as DTBT-1 and DTBT-2, respectively. Compared with DTBT-0, DTBT-1 and DTBT-2 exhibited remarkably enhanced fluorescent properties in both aggregated thin films and nanoparticles, demonstrating that the SIEE method could isolate the fluorophores effectively and then prevent their π–π stacking to achieve the impressive fluorescent properties. After proper surface modification, excellent water dispersibility, biocompatibility, and improved resistance to photobleaching were also achieved for highly emissiv...