Expression and significance of the protein and mRNA of metastasis suppressor gene ME491/CD63 and integrin alpha5 in ovarian cancer tissues.

Purpose: To investigate the expression and significance of the proteins and mRNA of metastasis suppressor gene Me491/cd63 and integrin a5 in ovarian cancer tissues. Methods: RT-PCR and in situ hybridization were used to detect the expression of the proteins and mRNA of ME491/CD63 and integrin a5 in normal ovarian tissues (Group I), ovarian benign tumor tissues (Group II), ovarian borderline tumor tissues (Group III) and ovarian cancer tissues (Group IV), and the correlation between the expression and the age of patient, degree of differentiation, lymphatic metastasis, stage and pathological type was analyzed. Results: There was a significant change in gene expression between the well and moderately differentiated tumors and poorly differentiated tumors. Gene expression in Groups III and IV was significantly weak, and significantly different from that in Group II and the early-stages of Groups III and IV. There was a significant difference in gene expression between each group. Conclusion: There is low expression of the proteins and mRNA of ME491/CD63 and integrin a5 in ovarian cancer. The lower the pathological differentiation is, the more significant the loss of expression is and the more likely metastasis is.