A validated RP-HPLC-PDA method for quantification of Eugenol in primed seeds of tomato, Brinjal, and Chilli using plant based Eugenol and commercial Eugenol

A laboratory experiment was conducted to quantify the eugenol in seeds primed with plant based eugenol (tulsi leaf extract, clove bud extract) and commercial eugenol, and also in the roots of resultant seedlings by developing and verifying a rapid and accurate Reverse Phase High-Pressure Liquid Chromatography method. The tomato, brinjal, and chilli seeds were primed (12hrs, 6hrs, and 12hrs respectively) with a standardized concentration of plant based and commercial eugenol. The different priming treatments such as T1-Control, T2-Hydropriming, T3-Purple tulsi extract (3%), T4-Clove bud extract (2%), and T5-Commercial eugenol (0.25%) were adopted in this experiment. The results revealed that the maximum quantity of eugenol was recorded in seeds as well as roots of tomato and brinjal which were primed with clove bud extract (2%), whereas in chilli, the seeds primed with commercial eugenol (0.25%) followed by clove bud extract (2%) recorded the maximum quantity of eugenol in seeds and roots of resultant seedlings, respectively. Eugenol content was absent in control and hydroprimed seeds and seedlings (root). The results also indicate that more than 90 per cent of eugenol was transferred from primed seeds to the roots of all three crops in the case of plant based eugenol.