Abstract 36: Concurrent Ultrasound Diagnosis and Treatment of Thrombosis Using Activated Platelet Targeted Theranostic Microbubbles

Molecular ultrasound imaging is an attractive non-invasive technology widely available for rapid clinical diagnosis. We hypothesized that thrombolytic drugs loaded microbubbles (MBs), which are selectively targeted to activated platelets, will allow high-resolution, real-time imaging of thrombosis, and at the same time offer potent thrombolytic efficacy without bleeding complications, and enable the immediate monitoring of success or failure of thrombolysis. Our therapeutic agents/imaging particles, targeted theranostic microbubbles (TT-MB), consist of a fusion construct that combines the fibrinolytic drug urokinase, echo-enhancing microbubbles for visualization by ultrasonography, and an activated-platelet-specific single-chain antibody for targeting specifically to thrombi. In the ferric-chloride induced carotid artery thrombosis mouse model, treatment with TT-MB significantly reduced thrombus size after 45 min, while no significant difference was observed in the MB that were targeted but without urokin...