THE USE OF COMPLEX OF MOLECULARGENETIC METHODS (PCRRFLP) TO STUDY OF SPOTTED FEVER GROUP RICKETTSIA

Restriction fragment length polymorphism analysis of PCR"amplified 590"bp gene fragment coding for the surface protein rOmpA was used for genotyping of 6 strains of spotted fever group (SFG) rickettsiae. These strains were isolated on the territory of Russia and Kazakhstan from differed species of ticks during 1984– 2000s. The PCR"fragments were cleaved with restriction endonucleases PstI and RsaI, and the digestion pat" terns were analyzed by 1 % agarose gel electrophoresis and compared with profiles of known species of SFG rickettsiae. The combination of the profiles obtained after digestion of the PCR product allowed for the differ" entiation of 2 groups’ rickettsiae (R. sibirica subsp. sibirica – R. sibirica subsp. BJ"90 и R. sp. RpA4 – R. sp. DnS14 – R. sp. DnS28).