Experimental study on synthesizing and appraising depreotide

Objective To synthesize depreotide which possesses a high affinity for the somatostatin receptor and contain a chelator for the radionuclide technetium-99m.Methods The cyclopeptide,cyclo-[(N-Me)Phe-Tyr-D-Trp-Lys-Val-Hcy],the linear peptide,and[ClCH2-CO.β-Dap-Lys-Cys-Lys.amide] were synthesised by Fmoc solid-phase synthesis.The cyclopeptide and the linear peptide were linked by liquid-phase synthesis.The product was isolated and purified by high performance liquid chromatography and was confirmed by mass spectrography.Results The molecular weight of synthesized product was 1358.The purity was 95.29%.The product was depreotide.Conclusion High purity depreotide is synthesized by Fmoc solid-phase synthesis and liquid-phase synthesis.