Translation of PVX RNA in vitro by wheat germ I. Characterization of the reaction and product size

Abstract Potato virus X (PVX) RNA was an efficient mRNA for in vitro translation by the wheat germ system. Optimal incorporation of labeled amino acids occurred in reaction mixtures (50 μl) containing 3 m M Mg 2+ , 70 m M K + , and 3–4 μg of RNA. The presence of PVX RNA stimulated amino acid incorporation up to 40 times over background levels. Addition of spermine or spermidine further stimulated amino acid incorporation nearly twofold. PVX RNA translation was not inhibited by adding double-stranded RNA from Penicillium stoloniferum . The largest of several polypeptides obtained from PVX RNA translation reaction mixtures had an apparent molecular mass of 110,000 daltons. No polypeptide that coelectrophoresed with native PVX coat protein subunits was observed even when heat-treated PVX RNA was used, or when the products from spermine-stimulated reactions were analyzed.