Abstract 12112: Genome-Wide Binding Profiles of c-Myc and RNA-Polymerase II Clarifies c-Myc-Mediated Circuit Pathway in RNA Metabolism in Cardiomyocytes

Background:c-Myc (Myc) transcription factor is rapidly induced in cardiomyocyte under adrenergic stimulation and has a functional property to strongly induce cardiomyocyte hypertrophy. Although the underlying mechanisms of pro-hypertrophic effect of Myc including the induction of protein and DNA synthesis have been extensively studied, the functional role for Myc in RNA metabolism in cardiomyocytes remains unclear. Methods:Global RNA production in rat neonatal cardiomyocytes with or without increasing amount of Myc protein were evaluated using 5-Ethynyl Uridine (EU) incorporation. Quantitative analysis using imaging cytometry demonstrated that adenoviraly transduced Myc significantly increased the amount of global RNA production in cardiomyocytes. To clarify the direct effect of Myc for mRNA transcription, we performed chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) in replicates and obtained genome-wide binding profiles of Myc and RNA-polymerase II (Pol II) in cardiomyocyt...