Dietary nucleoside-nucleotide mixture aggravates nasal allergic responses induced by toluene diisocyanate in mice

Abstract In this study we evaluated the effect of a dietary nucleoside-nucleotide mixture (NNM) on nasal allergy induced by toluene diisocyanate(TDI) in mice. Female ddY mice were randomized into two groups and fed a nucleic-acid free 20% casein (control) or this diet supplemented with NNM for 4 weeks. On the 4th week, mice were divided into 2 sub-groups; NNM sensitized, NNM- nonsensitized, control-sensitized and control-nonsensitized. The sensitized groups of mice were treated with two courses of intranasal application of 5% TDI in diethyl acetate for 5 consecutive days each time, separated by one week rest. Nonsensitized group of mice were similarly treated with vehicle. A week after the second sensitization all the treatment groups were provoked by applying 2.5% of TDI in vehicle. Nasal responses of sneezing, rhinorrhea, itching and snort at provocation, were scored for ten minutes. Degree of hair loss from the snout of mice was also scored for the length of the experiment. Lung and serum IgE were measured by ELISA. Results show that the sensitized groups of mice from each dietary group sneezed more frequently than the nonsensitized groups (p