Atividade antibacteriana de cimentos endodônticos puros e acrescidos de antibióticos contra duas linhagens ATCC de Enterococcus faecalis

Introducao: canais radiculares necrosados e nao tratados se caracterizam pela presenca de uma microbiota mista e polimicrobiana.O E. faecalis demonstra alta resistencia a medicamentos usados durante o tratamento endodontico, sendo um dos poucos microrganismos que tem demonstrado in vitro resistir ao efeito antibacteriano do hidroxido de calcio. Alem disso, e a especie bacteriana mais frequentemente isolada, com prevalencia variando entre 29% e 77% nos canais radiculares de dentes com insucesso endodontico. Objetivo: avaliar a atividade antibacteriana dos cimentos endodonticos MTA Fillapex, Sealapex, Sealer 26 e Endofill puros e acrescidos de amoxicilina e ciprofloxacina frente a duas linhagens ATCC de Enterococcus faecalis (ATCC 29212 e ATCC 4083). Metodo: os testes foram realizados pela tecnica de difusao radial dos cimentos aplicados diretamente no agar, impregnados em discos de papel aplicados sobre o agar e pelo teste de contato direto. Para a analise estatistica utilizaram-se os testes de ANOVA e Tukey com nivel de significância de 5%. Resultados e Discussao: os resultados mostraram que pela tecnica da difusao radial, considerando os cimentos puros, o Sealapex foi o unico que apresentou halo de inibicao frente as duas linhagens. Ja, quando da associacao da amoxicilina e ciprofloxacina o MTA Fillapex demonstrou os melhores halos de inibicao para as duas linhagens. Todos os cimentos tiveram a acao potencializada pela adicao dos antibioticos (p<0,05). Pela tecnica de difusao dos cimentos impregnados em discos de papel, o cimento Sealapex puro fresco revelou atividade antimicrobiana e tambem em todas as variaveis de tempo. Quando acrescidos os antibioticos, todos os cimentos tiveram a acao antimicrobiana potencializada pelas drogas, em todas as variaveis de tempo. Este mesmo resultado foi observado na tecnica do contato direto dos cimentos com ambas as linhagens. Conclusao: a atividade antibacteriana, frente ao E. faecalis, dos cimentos MTA Fillapex, Endofill, Sealer e Sealapex acrescidos os antibioticos amoxicilina e ciprofloxacina, e potencializada mesmo apos um longo periodo da presa dos cimentos. Introduction: necrotic root canals and untreated are characterized by the presence of a mixed and polymicrobial flora. E. faecalis show high resistance to drugs used during endodontic treatment, one of the few microorganisms which have shown in vitro to resist the antibacterial effect of calcium hydroxide. Furthermore, it is the bacterial species most often isolated, with a prevalence ranging between 29% and 77%, in root canals of teeth with endodontic failure. Objective: the aim of this study was to evaluate the antibacterial activity of the sealers MTA Fillapex, Sealapex, Sealer 26 and Endofill, pure and loaded with amoxicillin and ciprofloxacin against two ATCC strains of Enterococcus faecalis (ATCC 29212 and ATCC 4083). Method: the tests were conducted by means of radial diffusion technique of the cement applied in to the agar, impregnated paper discs applied on the agar and by the direct contact test. For the statistical analysis, the ANOVA and the Tukey?s test were used at a 5% significance level. Results and Discussion: results showed that, through the technique of radial diffusion, regarding the pure cements, the Sealapex was the only one that showed inhibition halo for the two strains. However, considering the association of amoxicillin and ciprofloxacin Fillapex, the MTA showed better inhibition zones for the two strains. All cements had their actions enhanced by with addition of antibiotics (p <0.05). Through the technique of diffusion of cements impregnated in paper discs, the Sealapex pure fresh cement revealed an antimicrobial activity and also in all the variables of time. When loaded with antibiotics, all cements had the antimicrobial action potentiated by drugs in all variables of time. The same result was observed in the technique of direct contact of cement with both strains. Conclusionn: the antibacterial activity against the E. faecalis, of the cements MTA Fillapex, Endofill, Sealer and Sealapex loaded with the antibiotics amoxicillin and ciprofloxacin, is enhanced even after a long period of use of cements.