Baculovirus-Derived HIV-1 Virus-Like Particles (VLP) Activate Dendritic Cells and Are Cross-Presented to Induce In Vitro T-Cell Response

Results the VLP-activated MDDCs show an enhanced Th1 and Th2-specific cytokine production and the effects of VLPs on MDDCs are, to some extent, mediated through intracellular Toll-like receptors signaling. The VLP-loaded MDDCs are able to induce a primary and secondary response in autologous T cells, using an in vitro immunization assay. Moreover, the genomic transcriptional profile of VLP-activated DCs show, by gene microarray analysis, the upregulation of several genes involved in the immune response.