[Study of aniline's penetration capability through rats' complete skin in vitro and normal lab gloves].

Objective To study the permeability of intact mouse abdominal skin to aniline and the protective capability of two typical lab gloves against aniline.Methods A Franz diffusion cell was used to perform in vitro transdermal absorption test and glove permeation test for aniline (0.102 mg/ml and 0.010 mg/ml).The permeabilities of intact mouse abdominal skin and gloves to aniline were measured by high performance liquid chromatography-diode array detection.Results The transdermal penetration of the two concentrations of aniline followed zero order kinetics within 12 h,exhibiting total aniline permeabilities within 24 h of 51.71％and 48.31％,respectively.The absorption liquid had an aniline concentration of at least 18 μg/L.The medical disposable latex glove could not stop the penetration of 0.010 mg/ml aniline,but the industrial natural latex glove could.Conclusion The penetration of 0.102 mg/ml and 0.010 mg/ml aniline through the mouse abdominal skin follows zero order kinetics within 12 h.The medical disposable latex glove cannot stop the penetration of 0.010 mg/ml aniline,but the industrial natural latex glove can. Key words: Aniline compounds; Skin absorption; Gloves, protection