Determination of the Contents of Organic Acids of Raspberry

As an edible Chinese herb,raspberry could improve overall energy,vitality aphrodisiac and immune booster.A method for determining organic acids in Raspberry by HPLC was developed and the best HPLC determination conditions with a C18 column were determined as follows: column temperature 30 ℃,mobile phase 0.05 mmol H2SO4,flow rate 0.5 mL/min,detection wavelength 210 nm and injection volume 10 μL.Under the detection conditions.five organic acids could be successfully separated with good linear relationships(correlation coefficients above 0.9998).The precision RSD,repeatability RSD and recovery rates were of 0.30-0.90%,0.42-3.69% and 97.86-102.35%,respectively.The method was convenient,rapid,accurate and applicable to determine organic acids in raspberry.