Multichromatic TTF staining characterizes cartilage matrix in osteoarthritis and bone development
2019
. Various histological staining methods have
been explored to detect the joint lesions in osteoarthritis
(OA), but these histological stains cannot
comprehensively present the comparatively complex
structures of articular cartilage in knee OA. In addition,
no integrated histological staining method can be used to
evaluate efficiently both the subzone region and matrix
composition in cartilage containing tissues. Therefore, in
this study, a novel multichromatic staining method
termed TTF staining, using Toluidine Blue (T),
Tartrazine (T) and Fast Green (F) sequential combined
staining for histological analysis, has been exploited to
characterize the changes of matrix components and
contents in cartilage during OA and in the bone
development. This specific TTF staining profile can be
used to differentiate the major compartments of knee
joint region, including the synovium, meniscus, multiple
subzones of cartilage and subchondral bone. An anterior
cruciate ligament transection induced OA model in rat
has been established to profoundly present the
alterations of glycosaminoglycans in cartilage
degeneration by TTF staining profile. The changes of
TTF staining profile in the chondrification and
ossification centers of the postnatal rat knee joint
indicate the developmental features of cartilage matrix
during the growth of bone. In summary, we have
developed an effective histological staining method that
enables us to identify the subzones of cartilage in detail
and to define the matrix features of bone development.
Therefore, finally using this new TTF staining method
may help us to exploit a histopathological grading
system to assess cartilage lesions in clinical disease.
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