Experimental study on the functional regulation of naringin in human periodontal ligament cells

PURPOSE:To detect the effect of naringin on human periodontal ligament cells'(hPDLCs) proliferation,bone formation and OPG mRNA expression.METHODS: hPDLCs were primarily cultured and identified in vitro through enzyme digestion combined tissue culture method.With 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)method,enzyme linked immunosorbent assay and RT-PCR,the hPDLCs' proliferation,alkaline phosphatase(ALP)activity,expression of collagen protein-Ⅰand OPG mRNA were observed after treatment with different concentrations(100,10,1.0,0.1,0.01mg/L)of naringin at different times.SPSS16.0 software package was used for statistical analysis.RESULTS: Primary cultured hPDLCs had good shape;Significant promotion of proliferation,ALP activity and collagen protein-Ⅰexpression of hPDLCs with naringin was found at the dose of 1.0 mg/L;and 1.0mg/L naringin regulated the expression of OPG mRNA in time-dependent manner.CONCLUSION: Naringin might significantly promote the proliferation of hPDLCs and conversion into the osteoblast.Supported by National Natural Science Foundation of China(30940092) and "13115" Major Scientific and Technological Innovation Special Projects of Shanxi Province(2009ZDKG-77).