sHRNA (short hairpin ribonucleic acid) for inhibiting expression of human oral cancer cell PRPS2 (phosphoribosyl pyrophosphate synthetase subunit II) as well as construction and application of carrier of sHRNA

The invention relates to the field of biological medicines, and in particular relates to an shRNA (short hairpin ribonucleic acid) for inhibiting expression of human oral cancer cell PRPS2 (phosphoribosyl pyrophosphate synthetase subunit II) as well as construction and application of carrier of the shRNA. The shRNA is shown in SEQ ID NO.1, a target sequence of the shRNA is as whon in SEQ ID NO:2, and is transcribed and generated through a DNA (deoxyribonucleic acid) template sequence shown in SEQ ID NO.3; the template sequence and a linear carrier are recombined, and are recombined with an hTERT (human telomerase reverse transcriptase) promoter sequence to obtain an shRNA eukaryotic expression plasmid for inhibiting expression of human oral cancer cell PRPS2. Verification indicates that the eukaryotic expression plasmid carrier can be used for stably and constantly reducing the expression level of mRNA (messenger ribonucleic acid) and protein of the PRPS2, and causing occurrence of cell prolifation inhibition, cell cycle repression and apoptosis, so that the defects of short acting duration, high toxicity and the like of in vitro chemically synthesized siRNA (small interfering ribonucleic acid) can be overcome.