Gq-Gi-PLCβ in cells: more than the sum of its reconstituted parts

Abstract The recent paper by Pfeil et al. , “Heterotrimeric G Protein Subunit Gαq Is a Master Switch for Gβγ-Mediated Calcium Mobilization by Gi-Coupled GPCRs”, opens another path from biochemical in vitro reconstitution to understanding the complex regulation of calcium signaling inside the cell.