[Effects of among compositions of Herba Ephedrae decoction on genic xpression of 5-lipoxygenase activating protein, IL-4 and leukotriene C4 in asthmatic mice].

Objective: To explore the regularity of recipe composition by observing inhibitory effects on the genic expression of 5-lipoxygenase activating protein,IL-4 and the leukotriene C4 in asthmatic mice.Method: The mice were challenged with OVA and administered ig with the Herba Ephedrae decoction(HED),separated compositions(2 500 mg·kg-1,calculated by Herba Ephedrae) and dexamethasone(2 mg·kg-1) respectively once daily for seven days.The real-tme fluorescence quantitative PCR method was employed to measure the contents of FLAP mRNA and IL-4 mRNA expressions in lung and the ELISA method was used to determine the content of LTC4 in the washing solution of pulmonary alveolus and bronchi.Result: In the lung of asthma mice,the expressions of FLAP and IL-4 and the content of LTC4 were significantly augmented compared with the control group.The HED and the separated compositions could suppress the expressions of FLAP and IL-4 and LTC4 release to a great extent in mice.Conclusion: The HED had the remarkable effects of antianaphylaxis asthma and the original formula HED worked best.These results confirmed the rationality and scientific level of HED.