Regulation of LPS-stimulated IL-10 secretion by PKC in macrophages.

IL-10 is a potent anti-inflammatory cytokine produced by a variety of leukocytes including macrophages. It is important to understand the regulation of IL-10 because of its ability to influence the immune response and suppress inflammation. Macrophages stimulated with LPS secrete small amounts of IL-10. However, when combined with FcγR ligation LPS induces the production of large amounts of IL-10 by macrophages. The signaling pathways responsible for LPS-stimulated IL-10 and the positive effect of the FcγR on LPS-stimulated IL-10 production are not well characterized. PKC is activated by LPS and that activation is altered by FcγR ligation in macrophages. This study evaluated the role of PKC in LPS-stimulated IL-10 secretion and the effect of FcγR ligation on LPS-stimulated IL-10 secretion by mouse peritoneal macrophages. Inhibition of PKC with Go6976 or Rottlerin decreased LPS-stimulated IL-10 secretion and rescued the effects of FcγR ligation. However, depletion of PKC-α and PKC-e isoforms with antisense...