A novel fluorescent turn-on probe for hydrogen peroxide based on carbon dots

Detection of hydrogen peroxide is of significant importance for biological assays, and fluorescence methods are intensively reported for this purpose. Due to the highly oxidative property of this species, usually fluorescence quenching is obtained during the interactions and decreased signals. Unlike carbon quantum dots (CQD)-based probes, it has been reported so far, adding H2O2 to the probe increases the fluorescence intensity (with excitation/emission maxima at 450/530 nm). The probe has a linear response range in the 3.00 μM to 300 mM with a detection limit of 1.00 µM (at S/N = 3). It was successfully applied to the determination of H2O2 in spiked to serum samples.