Antioxidant, anti-inflammatory and anti-tumor potential of supercritical carbon dioxide extracts from Ixeris chinensis

Supercritical carbon dioxide extraction was used to obtain effective extracts from Ixeris chinensis . Antioxidative activity, anti-inflammatorial properties and antitumor potential of the extract were studied in this research. Qualitative and quantitative analysis of the effective component of the I. chinensis extracts (IX) was performed using high-pressure liquid chromatography (HPLC) with an ultra violet (UV) spectrophotometer and mass detector. The results revealed that 2,2-diphenyl-1-picrylhydrazyl (DPPH ·) radical scavenging activity of 50% (IC 50 ) was 12.5 µg/ml. IX at a concentration of 350 µg/ml could prevent oxidative stress induced by hydrogen peroxide (H 2O2) and decrease the phorbol esters (PMA) induced THP-1 monocyte differentiation to 64.3%. HepG2 cells treated with IX at a concentration of 350 µg/ml caused a rapid induction of caspase3 activity and stimulated proteolytic cleavage of poly (ADPribose) polymerase (PARP) and induced apoptosis. Quantitative analysis showed that the content of apigenin and luteolin in the IX was 9.56 and 4.24 mg/g, respectively.