Скрининг сред с различным содержанием биогенных элементов для культивирования микроводорослей, ассоциированных с беспозвоночными Белого моря

The growth and biomass accumulation of three microalgal strains of Desmodesmus (Scenedesmaceae, Chlorophyceae), 1Рm66В, 2Cl66E, 3Dp86Е-1, isolated from White Sea benthic invertebrates were studied under conditions of batch culture in different standard (BG-11, Prat, Goldberg, Gromov, Tamiya, artificial sea water) and modified media. Culture condition and biomass accumulation were recorded as well as the uptake of nitrate and phosphate. Vigorous growth of the microalgae brought about a significant alkalization of the culture medium to pH 10. The most significant biomass accumulation was recorded in BG-11 (the complete medium and one with addition of artificial sea water), Tamiya and Prat media. Addition of the sea water did not affect the growth of Desmodesmus sp. in the nitrate-containing media although that maintained the growth of the microalgae in the nitrogen-lacking media without cell aggregation. The obtained results suggest the suitability of BG-11 medium for isolation and cultivation of both symbiotic and free-living microalgae. The Prat medium is more suitable for maintaining the microalgal strains in collection.