Effects of Ginkgo biloba tablet combination enalapril in renal interstitial fibrosis rats with unilateral ureteral obstruction

Objective To observe the preventive and therapeutic effects of Ginkgo biloba tablet combinaton enalapril in renal interstitial fibrosis rats.Methods Unilateral Ureteral Obstruction(UUO)model was induced by ligating the left ureter in rats.75 male sprague dawley rats were randomly divided into 5 groups:sham operation group,model group,control groups(enalapril group,ginkgo biloba tablet group,ginkgo biloba tablet combination enalapril group).Every group has 15 rats.Rats in treated group were differently injected ginkgo biloba tablet(300 mg/(kg·d) or enalapril(10 mg/(kg·d)or ginkgo biloba tablet combination enalapril.HE,The rats in sham and control groups were injected the same volume of normal water.Such were observed at 3th,7th and 14th day:pathological changes of the obstructed kidney and the changes of TGF-β1,NF-κB,α-SMA and nephridial tissue collagen protein/kidney weight.Results Massive inflammatory infiltrates and collagen expression in renal interstitial in the model group were observed.Compared with the sham operation group,percentages of area of TGF-β1,NF-κB and α-SMA expressions in the model group were markedly increased(P0.05).After intervention with treated group,the upregulation of the above-mentioned parameters were all significantly inhibited(P0.05).There was significant difference of intervention effects between ginkgo biloba tablet combination enalapril group and other-treated group(P0.05).There was no significant difference of intervention effects between enalapril group and ginkgo biloba tablet group(P0.05).Conclusion Ginkgo biloba tablet group can reduce inflammatory infiltrates,renal interstitium collagen protein content,and the degree of interstitial fibrosis.Ginkgo biloba tablet combination enalapril could significantly attenuates renal interstitial fibrosis.