Original Paper : Polymorphonuclear Leukocytes Adhesion onto Polymeric Charge Carriers

A series of PP-g-AAc-AAm of different charge levels were prepared as the polymeric charge carrier, and the adhesion of PMNs to the prepared surface as well as the effects of serum proteins on PMNs adhesion were studied systematically. The samples enhanced PMNs adhesion in the order of PP-g-AAc1-AAm4, PP-g-AAc2-AAm3, PP-g-AAc3-AAm2, and PP-g-AAc4-AAm1, eg., PMNs adhesion increased with increase in the positive charge of the substrates. From this, the charge interaction between the PMNs and the substrates seems to be the dominantfactor in the PMNs adhesion. However, the results from SDS-PAGE and the immuno-blotting showed that the adsorption of proteins, especially immune proteins, also increasedwith increase in the positive charge of the samples. In addition, PMNs adhered to the samples show the spreading behavior which is uncommon phenomenon for the cells tightly adhered by strong charge interaction. Therefore, it is concluded that the immune proteins bound to the samples enhances PMNs adhesion by increasing PMNs chemotaxis.