Effects of gene therapy by rAAV-Endostatin in treatment of bladder cancer： an experimental study in vivo

Objective To construct bladder cancer animal model using nude mice and study the therapeutic effect of recombinant adeno-associated virus （rAAV） -endostatin （ES） in vivo. Methods Cell suspension of T24 cells were subcutaneously injected into the right scapular region of nude mice. The nude mice bearing tumors were randomly divided into 3 groups ： rAAV-muhiple cloning site （ MCS ） group, rAAV-ES group and control group. After treatment for 4 weeks, the nude mice were killed and the tumor volume was compared. At the same time, blood ES concentration was assayed by enzyme-linked immuosorbent assay （ELISA）. Results At the 4th week after injection of rAAV-ES, the tumor volume in rAAV-ES group, rAAV-MCS group and control group was （0.75±0.08） cm3, （1.27 ±0.13 ） cm3 and （1.24±0. 17） cm3. The microvessel density （MVD） was （18. 72 ±2. 53）/HP, （52. 38 ±6. 46）/HP and （49.94 ±7.17）/HP, respectively. Conclusion rAAV-ES was successfully constructed, and it could inhibit angiogenesis. Key words: Bladder carcinoma;  Adeno-associated virus;  Endostatin;  Gene therapy