Роль оксида азота в механизмах действия монооксида углерода на сократительную активность гладкомышечных клеток аорты крысы

The effect of carbon monoxide (CO) on contractile reactions of smooth muscle cells (SMC) caused by depolarization (30mM KCl) and phenylephrine (PE) has been investigated by the mechanographical method. It is shown that the donor CO CORM-2, in experiments with of high potassium contraction in concentrations of 10-1000 µМ, and in cases phenilefrine-induced contractions (10 µМ) of SMC, of 1 µМ and above, caused a dose-dependent relaxation of vascular segments. Inhibition of NO-synthase and soluble guanylate cyclase (GC) weakened CO induced relaxation of the segments. With blocking potassium channels tetraethylammonium chloride and 4-aminopyridine relaxing effect СORM-2 on vascular segments virtually eliminated. These results may be the evidence of cooperation in the implementation of gazotransmitters gasotransmitters relaxating effect of CO on the level of soluble guanylate cyclase and potassium channels plasmalemma of SMC.