Preparation of 23-hydroxybetulinic Acid from the Roots of Pulsatilla chinensis

Objective: To isolate and purify high purity 23-hydroxybetulinic acid from the roots of Pulsatilla chinensis.Methods: By acid hydrolysis,silica gel column chromatographies and recrystalization methods,23-hydroxybetulinic acid was obtained from the roots of P.chinensis.Results: High purity 23-hydroxylbetulinc acid was isolated from the roots of P.chinensis,the contents is over 98.0%.Conclusion: The preparation process is feasible and convenient.High purity 23-hyroxybetulinic acid could be used in the quality control of the materials and medicament of 23-hydroxybetulinic acid.