Self interference digital holographic microscopy approach for inspection of technical and biological phase specimens
2011
Quantitative holographic phase contrast imaging enables high-resolution inspection of reflective surfaces and technical
phase specimen as well as the minimally invasive analysis of living cells. However, a drawback of many experimental
arrangements is the requirement for a separate reference wave which results in a phase stability decrease and the demand
for a precise adjustment of the intensity ratio between object and reference wave. Thus, a self interference digital
holographic microscopy (DHM) approach was explored which only requires a single object illumination wave. Due to
the Michelson interferometer design of the proposed setup two wave fronts with an almost identical curvature are
superimposed. This results in a nearly ideal pattern of spatial off-axis carrier fringes and a constant interferogram
contrast in the hologram plane. Moreover, the hologram evaluation with spatial phase shifting reconstruction algorithms
and Fourier transformation-based spatial filtering methods as well as the integration of DHM in common research
microscopes is simplified. Furthermore, the use of laser light sources with a short coherence length is enabled. The
applicability of the proposed self interference principle is illustrated by data from the analysis of technical and biological
phase specimens. The obtained results demonstrate that the method prospects to be a versatile tool for quantitative phase
contrast imaging.
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