GC-MS Analysis and HPTLC Fingerprinting Profile of Hydroalcoholic Extract of Polygonum barbatum Linn. Leaves

An important medicinal plant, Polygonum barbatum was undertaken for gas chromatography-mass spectrometry analysis and high performance thin layer chromatography finger print profile to investigate the chemical constituent present in the hydroalcoholic extract of Polygonum barbatum leaves. Gas chromatography-mass spectrometry analysis shown that twenty two compounds were identified and named from the extract. Among these constituents, terpenoids are the major constituents present in this extract. Other major constituents were present in the extracts were carbohydrate and fatty acids. High performance thin layer chromatography fingerprint analysis of hydroalcoholic extract of P. barbatum was carried out by using ethyl acetate-hexane (7:3 v/v) as a mobile phase. From the high performance thin layer chromatography analysis, peak numbers 4, 5 and 6 shown highest % of peak area of 29.79, 21.42 and 10.67, respectively at 366 nm.