Improvement of Epinephrine Autoxidation Method for Determination of SOD Activity and Its Influential Factors

Objective To increase the sensitivity of the epinephrine autoxidation method for the determination of superoxide dismutase(SOD) activity.Methods The new wavelength and reaction system for the SOD activity determination were adopted.The influential factors including concentration of epinephrine,pH,temperature andmetal ion were studied for their influnce on the autoxidation rate.Results The sensitivity determined under thewavelength of 325nm was higher than that under 480nm.With the increase of substrate concentration,pH andtemperature,the autoxidation rate rose accordingly.Measuring the SOD standard preparation with this improvedmethod,the average recovery rate and coefficient of variation(CV) in batches are 93.32% and 7.71%,respectively,and maximal inhibition rate is 70.30%.Conclusion The improved determination system is more sensitive,repro-ducible and exact.