Analysis of distribution and drug resistance spectrum of bacteria and candida isolates from midstream voided urine, blood, sputum of denile patients.

Objective To investigate distribution and drug resistance spectrum of clinical bacteria and candida isolates from midstream voided urine,blood,sputum from senile patients in local region for using antibiotic reasonably. Methods Most bacteria isolates were identified with BD Phoenix,a few were identified with handy work and K-B method. Candida isolates were identified with color display plate and K-B method. WHONET5.4 was applied for analysis. Results The common isolates from midstream voided urine from elder patients were E. coli,C. albicans,and K. pneumoniae. The common isolates from blood were E. coli,K. pneumoniae,and P.aeruginosa. The common isolates from sputum were C. albicans,A. baumannii,P.aeruginosa,and S. aureu. The incidences of E.coli isolates producing extended spectrum beta -lactamase (ESBLs)from midstream voided urine,blood,sputum were 39.5%,34.0%,and 60.4% respectively. The methecillin resistant rates of S. aureu,S. epidermidis,and S. haemolyticus from sputum were 71.4%,88.5%,and 90.0% respectively. The resistant rates of gram negative bacillus from midstream voided urine and blood to imipenem,cefoperazone/sulbactam,amikacin, piperacillin/Tazobactam,and ceftazidime were lower,resitance of gram negative bacillus from sputum to cefoperazone/ sulbactam and imipenem was lower. Gram positive coccus,to vancomycin and teicoplanin were 100.0% ,resistant rates of other antibiotics were high. In Candida isolates,resistant rates of amphotericin B and nystain were 0.0%. Conclusion The incidences of candida,the production ratios of ESBLs of E.coli and K. pneumoniae isolates,carbopenem resistant rates of non -ferment gram negative bacillus,and methicillin resistant rates of staphylococci from midstream voided urine,blood, sputum from elder patients were high. It is important to enforce the rational use of antibiotic.