Protein tyrosine phosphatase 3 improves the invasion of LoVo cells through induceing Ca(2+) -activated K+ channels 4 of tumor associated macrophages

Objective To investigate the interaction between colon cancer cells and tumor associated cells (M2),and it' s influence to tumor cells' function.Methods LoVo cells [transfected with protein tyrosine phosphatase 3 (PRL-3)] and M2 cells were cocultured,Western blot were used to examine Ca2 +-activated K + channels (KCNN4) expression of M2 cells.And the invasion of LoVo cells also be detected.Results Used by Western blotting it is found out that KCNN4 of M2 cells is improved after cocultured with PRL-3 LoVo cells.Besides,coculture also improves the invasion of LoVo cells(3367 ± 135 vs.1442 ± 89,P ＜ 0.05).When KCNN4 expression was blocked,the invasion of LoVo cells is reduced (1388 ±-87 vs.2893-± 163,P ＜0.05).Conclusion PRL-3 improves the invasion of LoVo cells through upregulating KCNN4 of M2 cells in the colon cancer microenvironment. Key words: Protein tyrosine phosphatase 3; Tumor associated macrophage; Ca2+-activated K + channels 4