C60 Fullerene Derivative： Radioprotective Properties and a Toxicity Test in Vivo ＆ in Witro

The scope of use of fullerenes and their derivatives, thanks to their material and bonding properties, is increasing, including their application in biological systems. The possibility of their use as substances affecting resistance to the effect of ionising radiation was examined experimentally in vivo in individual of Danio rerio in juvenile age (2.5 months) without differentiated sex and in vitro in tissue cultures in primoculture of guinea pig kidney cells and in melanoma cells B16-F10, parallel to the toxicity tests. In order to achieve the accessibility of fullerenes for living organisms the reaction of C60 fullerene with peracetic acid and followed by hydrolysis of oxo-fullerene prepared in water-soluble fullerene derivative characterised by its Fourrier transformation infra-red spectrum (FTIR), and in its solution at a concentration of 147 mg/L individuals of the juvenile form of Danio rerio can survive for a long time without signs of toxic effects. A radioprotective effect was shown in fish after their five-day stay in its solution. The period of survival increases after radiation by 10 to 70 Gy doses and up to 70%, and the LD50 value for various periods of survival roughly doubles. The effect shown is preventive and is probably associated with the ability of fullerenes to be able to eliminate free radicals and oxidants appearing by water radiolysis. Unlike fish, the prepared derived fullerene solutions for tissue culture in vitro are toxic in unit concentrations of up to tens of mg/L, whereas free cells are roughly 5 times more sensitive. No protective effect was observed in tissue cultures exposed briefly before radiation to the effects of the fullerene derivate solution. This may be the consequence of brief contact with fullerene nanoparticles before radiation when there was no time for them to penetrate the cells.