Influence of anti-microbial composition based on calcium alginate on museum strains of microorganisms

Annotation. In the context of antibiotic resistance development, the search for the new biologically active substances for the prevention and Treatment of Infectious Diseases is an important part of pharmaceutical research. The aim of the study was investigating the effect of antimicrobial composition based on calcium alginate on the museum strains of microorganisms. Standard strains of Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Enterococcus faecalis were used for the study. The investigated antimicrobial composition was made up of decamethoxine (0.2–0.5%) and calcium alginate as a film-forming agent. Sensitivity studies of museum strains of microorganisms were performed by disco-diffusion method according to the generally accepted method. The result of antimicrobial activity was evaluated after 24 and 48 hours. Statistical analysis of the obtained results was performed using standard software packages “STATISTICA +” and “Microsoft Excel 2010”. It is established, that the antimicrobial composition based on calcium alginate has a higher activity against gram-positive microorganisms, compared to the gram-negative strain. For 24 hours, the composition is not inferior to the antimicrobial action of a solution of decamethoxine relative to all strains of microorganisms. Moreover, after 48 hours of incubation the investigated composition retains antimicrobial action against museum strains of microorganisms.