MECHANISM OF ANTICANCER CHINESE MEDICINE "CANING" AGAINST OSTEOCARCINOMA

Objective To evaluate the effect of Chinese medicine “caning” by antiapotosis gene fas so as to detect the mechanism of treating cancer. Method We observed the inhibitive effect of drug-containing serum to human osteoma cell line by MTT colorimetry and its antiapoptosis effect of fas gene by PCR assay. Result It indicated that the fas activity of the Chinese herb group was dramatically higher than that of the control. It showed that the inhibition ratio of cell growth was positively related to the concentration and the time of the drug-containing serum, p0.05. It worked the best in the 15% drug-containing serum. It showed that “canning” can promoted the fas activity of the MG-63 cells in vitro Conclusion Anticancer drug “canning” induced cell apoptosis by enhancing the fas gene.