Review Article Extracellular/circulating microRNAs and their potential role in cardiovascular disease

microRNAs (miRs, miRNAs) are small non-coding RNAs that regulate hundreds of gene expression. Numer- ous studies have demonstrated that miRNAs are not only found intracellularly, but also detectable outside cells, in- cluding various body fluids (i.e. serum, plasma, saliva, urine, breast milk, and tears). Interestingly, ~90% of extracellu- lar miRNAs are packaged with proteins (i.e. Ago2, HDL, and other RNA-binding proteins) and ~10% are wrapped in small membranous particles (i.e. exosomes, microvesicles, and apoptotic bodies). It is believed that these extracellu- lar miRNAs mediate cell-to-cell communication. Recent studies further indicated that the level and composition of these extracellular/circulating miRNAs correlated well with disease or injurious conditions. Uncovering the potential role of extracellular miRNAs in the heart is just emerging. This review will highlight recent exciting findings in the regu- lation of miRNA biogenesis and secretion, their functional roles in paracrine signaling, and the potential as non- invasive biomarkers for cardiovascular disease.