Heat shock proteins and antioxidants as mechanisms of response to ivermectin in the dung beetle Euoniticellus intermedius

Abstract Ivermectin is the most common antiparasitic drug used in livestock in many regions of the world. Its residues are excreted in dung, threatening non-target fauna such as dung beetles, fundamental for cleaning dung in pastures. However, it is unclear which are the physiological mechanisms used by dung beetles to cope with ivermectin. Here we evaluated experimentally the physiological responses of the dung beetle Euoniticellus intermedius to ivermectin-induced stress. We measured metabolic rates, heat shock protein 70 (Hsp70) expression, antioxidant capacity, and oxidative damage in lipids in both males and females exposed to a sublethal dose. Compared to control beetles, ivermectin-treated males and females had increased metabolic rates. Moreover, ivermectin-treated females increased their expression of Hsp70 whereas males increased their antioxidant capacity. No changes in the levels of oxidative damage to lipids were detected for either sex, suggesting a process of hormesis, such that exposure to a moderate concentration of ivermectin could stimulate the action of a protective mechanism against oxidative stress, that differs between sexes. However, it does not exclude the possibility that damage to other biomolecules might have occurred. Sexual differences in physiological responses can be interpreted as the result of hormonal differences or life-history trade-offs that favor different mechanisms in females and males. Hsps and antioxidants are involved in the physiological response of beetles to ivermectin and may be key in providing resistance to this contaminant in target and non-target species, including dung beetles.