Identification and characterization of a selective radioligand for melanin-concentrating hormone 1-receptor (MCH1R).

Abstract We have developed and characterized [ 35 S] 4a as a potent and selective radioligand for melanin-concentrating hormone 1-receptor (MCH1R). Compound [ 35 S] 4a showed appreciable specific signals in brain slices prepared from wild-type mice but not from MCH1R deficient mice, confirming the specificity and utility of [ 35 S] 4a as a selective MCH1R radioligand for ex vivo receptor occupancy assays.