T cells from treated CMTB mice fail to expand upon infection.

(A) Following antibiotic treatment to clear CMTB as described in the main text, the fraction of CD3+CD8+TB10.4+ T cells in the lung vasculature and parenchyma, as determined by i.v. labeling with anti-CD45-PE antibody was measured by flow cytometry 10 days following aerosol challenge with 50–100 CFU of Mtb H37Rv. Statistical significance was determined by Student’s t-Test. Error bars represent the mean and SEM (B) The fraction of CD3+CD8+TB10.4+ T cells in whole lung homogenates of CMTB and control mice prior to aerosol challenge with 50–100 CFU of Mtb H37Rv and at 10 days following challenge was determined by flow cytometry. Statistical significance was determined by paired t-test. Error bars depict the mean and SEM. (n = 3–5 mice per group). (See S14 Fig for gating strategy.) (PDF)