一锅法制备聚邻氨基酚－纳米金－酶膜用于葡萄糖安培传感

We report on the one-pot preparation of high-performance glucose enzyme electrode through the co-electrode position of o-aminophenol (OAP) polymer and oAP oligomer (oAPO)-Au nanoparticles (AuNPs)-glucose oxidase (GO(subscript x)) nanocomposites, which are prepared via the redox reaction of HAuCl4 and oAP aqueous solution in the presence of GOx. The as-prepared biosensor is several folds enhanced in the sensitivity and one-magnitude lowered in the detection limit, as compared with those prepared by the conventional electropolymerization protocol and the similar protocol using K3Fe(CN)6 as the oxidant. Additionally, the proposed biosensor possesses rapid response, satisfactory anti-interference ability, reproducibility, and storability. The proposed protocol is recommended to prepare other multifunctional nanocomposites and may find wide applications in the biosensing and biomaterials fields.