SPECTROSCOPIC DETERMINATION OF TOTAL PHENOL AND FLAVONOID CONTENTS OF CITRUS LIMON PEEL FROM NORTH EASTERN REGION OF INDIA

Citrus fruit plants are valuable sources of phenolic and other biologically active compounds.  Phenolic compounds have been reported to have multiple biological effects. In this paper quantitative determination of phenols and flavonoids of citrus limon peel extracts was carried out using spectrophotometric method. The plant material was collected from Assam, north-eastern region of India. Total phenolic content was determined by Folin-Ciocalteau method and aluminium chloride method was used for flavonoid determination. Methanol and ethanol extracts of ripe and unripe peel of the plant contain high amount of phenol contents compared to acetone and ethyl acetate extracts. Methanol extract contains maximum and the ethyl acetate shows minimum amount of flavonoid contents. However, all the extracts show significant results. Key Words: Citrus limon, Phenol, Flavonoid, Catechol and Quercetin.Â