Method of preparing low-molecular chondroitin sulfate for treating myocarditis

Disclosed is a method of preparing low-molecular chondroitin sulfate (CS) for treating myocarditis, the steps comprising: fermenting raw material through an arthrobacter sulfonivorans fermentation liquor, and conducting enzymolysis treatment on CS in the raw material to obtain low-molecular CS; the content of the CS disaccharide in the prepared low-molecular CS is greater than 97%, and the molecular weight is 450-480; the content of CS A is 70-95%, and the content of CS C is 5-25%. The preparation method is easy to conduct, and has a short cycle and low production cost, and the low-molecular CS has a good treatment effect and high purity, can be used for large-scale industrial production, and has a significant repair effect on myocardial cells in rat experiments.