Determination of Folic Acid in Serum by SPE and High Performance Liquid Chromatography with Photochemical Spectrofluorimetry

This paper described a combined high-performance liquid chromatography (HPLC) -photochemical spectrofluorimetry/solid-phase extraction (SPE) procedure for determining the concentration of unmetabolized folic acid in human serum. After being extracted by SPE (C18-H) column, the serum samples were irradiated under the UV lamp for 11 h, separated on a C18 HPLC column with methanol/water as the mobile phases and determined by a fluorescence detector. Fluorescence excitation and emission wavelengths were at 280 and 443 nm, respectively. The linear range was 1.9 ~ 8.0 ng/mL. The recovery was from 88.9 to 123.6 % with RSD in the range of 4.1–6.4 %.