Therapeutic effects of benthiactzine against respiratory failure induced by cholinesterase inhibitor in rats

Objective To investigate the therapeutic effects of benthiactzine against respiratory failure induced by cholinesterase inhibitor dimethyl dichloro-vinyl phosphate (DDVP) in rats. Methods Forty-five male Wistar rats were divided into five groups randomly: control group,model group,and benthiactzine 0.5,1.0,2.0 mg/kg treatment groups (each n=9). Rats were treated with DDVP by intraperitoneal injection to reproduce respiratory failure model. The symptoms,respiratory rate (RR),blood gas analysis,electrolyte and plasma superoxide dismutase (SOD),malondialdehyde (MDA) and the pathological changes were observed before poisoning,during respiratory failure,and in different periods after the treatment. Results In rats with respiratory failure induced by DDVP,cyanosis and convulsion occurred in all groups. The success rates in three benthiactzine groups were 66.7% (6/9),77.8% (7/9) and 88.9% (8/9). The rats of benthiactzine treatment groups recovered in 15 minutes after treatment and returned to normal state in 30 minutes. RR also returned to normal in 30 minutes. When respiratory failure occurred,arterial oxygen partial pressure (PaO2),arterial oxygen saturation (SaO2) and plasma SOD were decreased,plasma MDA was increased,and mixed acidosis was found. Thirty minutes after the treatment of benthiactzine,all above parameters in three groups returned to normal (all P<0.01). In respiratory failure rats,pathological examination of lung tissue revealed dilatation of pulmonary vessels with aggregation of erythrocytes,widening of alveolar space with presence of red blood cells in alveoli with heavy infiltration of inflammatory cells,and pulmonary edema and hemorrhage. The lungs of rats treated with benthiactzine showed less intense pathological changes. Conclusion The new medicine against poisoning benthiactzine can be a favourable drug against respiratory failure induced by orgnaophosphorus pesticides. Key words: benthiactzine; cholinesterase inhibitor; orgnaophosphorus pesticide; poisoning; respiratory failure