MicroRNA Analysis from Acute to Convalescence in Crimean Congo Hemorrhagic Fever.

Crimean Congo hemorrhagic fever (CCHF) is one of the most important viral infections and is caused by Crimean Congo hemorrhagic fever orthonairovirus (CCHFV). Severity of CCHF can vary from a mild and nonspecific illness, to a severe disease with fatal outcomes. MicroRNAs (miRNAs) have increasing impact on the different pathways of viral infections. Within the transition process from acute phase to convalescence with 18 CCHF patients, we investigated the impacts on miRNA via microarray for the first time. We also compared miRNA gene expression within 16 severe and 15 mild cases. We identified Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) pathways associated with significant miRNAs utilizing DIANA TOOLS mirPath v.3. In this study miR-15b-5p and miR-29a-3p were significantly downregulated in statistical terms; miR-4741, miR-937-5p, miR-6068, miR-7110-5p miR-6126 and miR-7107-5p were upregulated in acute cases in comparison with convalescent patients (p≤0.05). 28 miRNAs (8 downregulated, 20 upregulated) were differentially expressed in severe CCHF patients compared with mild cases (p≤0.05). While miR-6732-3p, miR-4436b-5p, miR-483-3p, miR-6807-5p had the highest downregulation; miR-532-5p, miR-142-5p, miR-29c-3p and let-7f-5p had the highest upregulation in severe patients in comparison with mild cases. Consequently, we determined that CCHF induced miRNAs are associated with antiviral and proinflammatory pathways in acute and severe cases. In comparison with convalescence, these miRNAs in acute period may be therapeutic targets. This article is protected by copyright. All rights reserved.