Seedling Growth of Agronomic Crops in Crude Oil Contaminated Soil

Phytoremediation of hydrocarbon-contaminated soil shows promise as a low-cost alternative to most remediation methods. This study evaluated seedling growth of six crop species in crude oil contaminated soils. The experiments were conducted in a greenhouse. Weathered crude oil was added to an Ipava silt loam soil at the rate of 0 (control), 10, 50 and 100 g of crude oil kg -1 of soil, which was then placed into pots. Irrigation was used to maintain soil moisture at approximately field capacity. Five seeds of Zea mays, Melicago saliva, Lolium perenne, Triticum aestimm, Glycine max or Vicia villosa were sown per pot. The experimental design was completely randomized with five replications per treatment. Germination and seedling height data were recorded on day 7, 14, 21 and 28. Plants were harvested on day 28, separated into shoots and roots and dried to measure biomass. Analysis of variance was used to determine treatment significance. Significant treatment mean values were separated using Tukey's Honestly Significant Difference Test. Based upon percent emergence and plant biomass production in contaminated soil, Z. mays and G. max seedlings show the greatest potential to enhance remediation.