Development of Wheat-alien Lines with Added Leymus racemosus Chromosomes and 6VS/6AL Translocation Chromosomes

By chromosome C_banding and bi_color fluorescence in situ hybridization (FISH) using digoxigenin_labelled total genomic DNA of Leymus racemosus (Lam.) Tzvel. and biotinylated total genomic DNA of Haynaldia villosa (L.) Schur as probes, three wheat_alien lines with L. racemosus Lr.7 addition and H. villosa 6VS/6AL translocated chromosomes, and eight lines with L. racemosus Lr.14 addition and H. villosa 6VS/6AL translocated chromosomes were respectively identified from DALr.7×T6VS/6AL (93G51_4×P64) and DALr.14 ×T6VS/6AL (94G15×P64) F 2 or F 3 hybrids. Fluorescein_isothiocyanate_conjugated avidin and rhodamine_conjugated sheep anti_digoxigenin Fab fragment were used in bi_color FISH detection. The chromosomes of L.racemosus and 6VS fragment of H. villosa were simultaneously detected by their red and green fluorescence. Powdery mildew and scab resistance were also evaluated. The result showed that the obtained plants had high resistance to these two diseases. The potential usage of bi_color FISH in identifying chromatin of L.racemosus and H.villosa was discussed.