ALL IN ONE LATERAL-FLOW CHIP FOR ARRAY IMMUNOASSAY

We integrated an antibody array, a lateral flow channel and power-free pumping functions in one chip to realize a rapid and easy surface plasmon resonance (SPR) immunoassay technique. This one-time-use chip was composed of four injection-molded polymeric components. A continuous and constant liquid flow was realized by balancing flow resistance and capillary force. Simply by injecting a few microliters of sample solution onto the chip, we were able to detect kinetic reactions from 13 antibodies simultaneously within 10 min. even when the antigen was in real milk samples. This constitutes the first step towards fast screening for pathogenic toxigenicity.