Zellteilung und Makromolekülsynthesen in auswachsenden Sporen einer temperatursensitiven filamentösen Mutante von Bacillus subtilis
1982
A temperature sensitive mutant of Bacillus subtilis SB 19, strain ts 33-6 was characterized. This strain grows at 46°C (restrictive temperature) with reduced intensity without septation processes. Under restrictive conditions DNA- and RNA-synthesis are remarkably reduced. DNA, however, is synthesized continuously under restrictive conditions causing the formation of multinuclear filaments. Septation, induced at permissive temperature, is not prevented under restrictive conditions. That means that under restrictive conditions initiation of septation is blocked whereas formation of septa can be observed. Shift-up experiments have shown that the initiation of septation processes occurs at an early stage of cell cycle.
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