Chapter 13 – Detection of Foodborne Pathogens Using DNA Arrays

Detecting foodborne pathogens as quickly and reliably as possible is a matter of public safety. In the last two decades, the field of food microbiology has evolved with increasing speed, also thanks to genome sequences available for many of the pathogens involved. In this chapter, conventional methods, analytical techniques, and recent developments in food pathogen array-based detection, identification, and quantification are described. We report the main applications involving the use of DNA microarrays as detection tools. The chapter, initially, focuses on the most recent developments and experimental results of more traditional array technologies, then new biomolecular techniques, such as integrated platforms for the miniaturized detection of pathogens, are discussed with the description of some interesting approaches and innovative applications. A final paragraph, dedicated to the latest developments and trends in the “omics” field, suggests new opportunities to solve old food safety problems.